Table 2.

Mutant Pho4 protein lacking the Pho2 interaction domain (Pho4Δint) can activate UASp2 but not UASp1

Reporterβ-Galactosidase activity (U)a
Pho4ΔintWild-type Pho4
PHO5-lacZ 55920
UASp1CYC1-lacZ <5622
UASp2CYC1-lacZ 99199
  • a The activity of the PHO5promoter and of heterologous promoter constructs containing individualPHO5 UAS elements was measured in strains expressing either wild-type Pho4 or Pho4Δint. Activity values with the parent vectorCYC1-lacZ were <5U.