Table 2.

Viability of transfected NIH 3T3 cellsa

Plasmid construct104 Cells at:
5 Days7 Days
RcCMV/pBabe5 ± 117 ± 3
Stat3/pBabe6 ± 119 ± 4
Stat3Y/pBabe7 ± 218 ± 3
Stat3DB/pBabe7 ± 123 ± 5
RcCMV/pBabe/v-src 3 ± 118 ± 3
Stat3/v-src 3 ± 122 ± 4
Stat3Y/v-src 4 ± 223 ± 3
Stat3DB/v-src 3 ± 221 ± 3
Stat3S/v-src 4 ± 122 ± 3
Stat3Y/v-src 3 ± 123 ± 3
  • a Cells were transfected with a 10:1 ratio of Stat3-based constructs to v-src, as described in the legend to Fig. 3A. Cells (5 × 105) were plated onto selective media and counted 5 and 7 days later with a Coulter counter; counts were confirmed by Trypan blue exclusion and counting with a hemacytometer. Values are averages of three independent transfections ± standard deviations.