Table 1.

Contribution of the individual Pho4 binding sites toPHO5 promoter activity

Promoteraβ-Galactosidase activity (%)b
Wild type100
UASp1-mut14
UASp2-mut7
Pho4 Site3-mut71
UASp1-mut + UASp2-mut1
UASp1-mut + Pho4 Site3-mut10
  • a Pho4 binding sites at thePHO5 promoter were mutated as shown in Fig. 1.

  • b Activities of the mutated promoters are expressed relative to the activity of the wild-type promoter.