Table 1.

Colony forming by NIH 3T3 cells transfected with various plasmid constructsa

Plasmid constructNo. of NIH 3T3 colonies at ratio ofb:No. of v-src-transformed NIH 3T3 colonies
1:21:10
RcCMV0051 ± 7
Stat300183 ± 15
Stat3Y0022 ± 5
Stat3DB0027 ± 4
Stat3S0020 ± 3
v-src/RcCMV37 ± 532 ± 3 NDc
v-src/Stat3171 ± 16105 ± 5ND
v-src/Stat3Y18 ± 57 ± 2ND
v-src/Stat3DB21 ± 72 ± 1ND
v-src/Stat3S16 ± 31 ± 1ND
v-Ha-ras Leu61 30 ± 516 ± 3ND
v-ras/Stat333 ± 617 ± 4ND
v-ras/Stat3Y36 ± 415 ± 4ND
v-ras/Stat3DB32 ± 315 ± 2ND
v-ras/Stat3S38 ± 616 ± 4ND
  • a NIH 3T3 and v-src-transformed NIH 3T3 cells were transfected with various combinations of RcCMV (control vector), Stat3, Stat3Y, Stat3DB, Stat3S, pBabe/v-src, and v-Ha-ras Leu61 and plated into soft agar as described in the legends to Fig. 2, 3, and 5.

  • b Ratios are of v-src or v-ras to RcCMV Stat3-based constructs.

  • c ND, not done.