Table 4.

Localization of NPc-ARD fusion proteins

Cell line and protein or fusion proteinaNbN/CcCd
WT+/+ 3T3
 NPc<11090
 NPc–MAD3-ARD27658
 NPc–53BP2-ARD40537
 NPc–GABPβ-ARD404614
 NPc–Notch1-ARD196021
p50−/− 3T3
 NPc<1991
 NPc–MAD3-ARD48475
 NPc–53BP2-ARD46513
 NPc–GABPβ-ARD533710
 NPc–Notch1-ARD125335
p65−/− 3T3
 NPc1495
 NPc–MAD3-ARD58393
 NPc–53BP2-ARD32608
 NPc–GABPβ-ARD414316
 NPc–Notch1-ARD73657
  • a NPc or NPc-ARD fusion proteins containing an N-terminal c-Myc-derived epitope tag were expressed in the indicated 3T3 mouse fibroblasts. The cellular localization of each fusion protein was determined by using anti-myc IgG. At least 100 cells that were positive for expression of each fusion protein were scored.

  • b The percentage of cells that displayed predominantly nuclear staining is expressed relative to the total number of cells that displayed staining for the respective NPc fusion protein.

  • c The percentage of cells that displayed predominantly whole-cell staining is expressed relative to the total number of cells that displayed staining for the respective NPc fusion protein.

  • d The percentage of cells that displayed predominantly cytoplasmic staining is expressed relative to the total number of cells that displayed staining for the respective NPc fusion protein.