Table 7.

Expression of fus2::lacZin wild-type and spa2Δ cells treated with different concentrations of mating pheromone

Concn of α-factor (nM)Relative β-Gal activity (mean ± SD)
SPA2 cellsspa2Δ cellsspa2(1-2,116-1466) cells
01.00 ± 0.080.32 ± 0.030.34 ± 0.02
0.61.06 ± 0.060.48 ± 0.060.52 ± 0.02
66.25 ± 0.214.02 ± 0.444.54 ± 0.17
6037.56 ± 0.4423.40 ± 2.1521.19 ± 1.69
6,000831.25 ± 14.58633.33 ± 29.17627.08 ± 14.58
  • a SPA2, spa2Δ, andspa2(1-2,116-1466) cells containing afus2::lacZ reporter were grown to early log phase (OD600 = 0.3) and treated with the indicated concentrations of α-factor mating pheromone for 1.5 h. Cells were collected, and β-Gal activity was measured as nanomoles ofo-nitrophenyl-β-d-galatopyranoside hydrolyzed per minute per milligram of total yeast proteins. The activities presented here are fold differences relative to the activity of vegetative SPA2 cells without pheromone treatment. Thespa2 mutants (Y2015 and Y2016) used in this experiment are direct derivatives of a SPA2 fus2::lacZstrain (Y2014 [Table 1]). Two independent isolates each ofspa2Δ and spa2(1-2,116-1466) cells were analyzed here.