Table 2.

Plasmids used in the two-hybrid analyses

Fusion and plasmidaDescriptionSource or reference
LexA fusions
 pB342 (pSH2-1)Vector; pLexA(1-87)/2μm/HIS3 27, 32
 pB551*pLexA::SPA2(1-1466)/2μm/HIS3 K. Madden
 pB552pLexA::SPA2(1-698)/2μm/HIS3 This study
 pB553pLexA::SPA2(512-698)/2μm/HIS3 This study
 pB554pLexA::SPA2(512-1118)/2μm/HIS3 This study
 pB555pLexA::SPA2(743-1466)/2μm/HIS3 This study
 pB556pLexA::SPA2(743-1118)2μm/HIS3 This study
 pB557pLexA::SPA2(1-530)/2μm/HIS3 This study
 pB558pLexA::SPA2(1-488)/2μm/HIS3 This study
 pB559pLexA::SPA2(112-698)/2μm/HIS3 This study
 pB560pLexA::SPA2(112-530)/2μm/HIS3 This study
 pB561pLexA::SPA2(281-698)/2μm/HIS3 This study
 pB562pLexA::SPA2(281-530)/2μm/HIS3 This study
 pB563*pLexA::SPA2(375-698)/2μm/HIS3 This study
 pB564pLexA::SPA2(1-150)/2μm/HIS3 This study
 pB565pLexA::BUD6(1-788)/2μm/HIS3 This study
 pB566pLexA::BUD6(272-788)/2μm/HIS3 This study
 pB567pLexA::BUD6(358-768)/2μm/HIS3 This study
 pB568pLexA::STE11/2μm/HIS3 14a
 pB46*pLexA::CIK1(20-466)/2μm/HIS3 62
GAL4 AD fusions
 pB282 (pACTII)Vector; pAD/2μm/LEU2 22a
 pB581pAD::PEA2(1-420)/2μm/LEU2 This study
 pB582pAD::SPA2(1-150)/2μm/LEU2 This study
 pB583* (p831)pAD::BUD6(275-788)/2μm/LEU2 26
 pB584* (p688)pAD::BUD6(478-788)/2μm/LEU2 26
 pB585pAD::BUD6(358-768)/2μm/LEU2 This study
 pB586*pAD::ACT1(1-375)/2μm/LEU2 This study
 pB587*pAD::BEM1(1-551)/2μm/LEU2 This study
 pB588pAD::MKK1(1-508)/2μm/LEU2 This study
 pB589pAD::MKK1(214-508)/2μm/LEU2 This study
 pB590pAD::MKK1(1-214)/2μm/LEU2 This study
 pB591pAD::MKK1(1-179)/2μm/LEU2 This study
 pB592*pAD::MKK2(1-508)/2μm/LEU2 G. Paravicini
 pB331pAD::SLT2(1-484)/2μm/LEU2 16
 pB593pAD::PBS2(1-668)/2μm/LEU2 This study
 pB594 (pSL2091)pAD::STE11/2μm/LEU2 67a
 pB595 (pSL2165)pAD::STE7(1-515)/2μm/LEU2 67a
 pB596 (pSL2170)pAD::STE7(172-515)/2μm/LEU2 67a
 pB597pAD::STE7(1-172)/2μm/LEU2 This study
 pB598*pAD::STE7(1-136)/2μm/LEU2 This study
 pB599 (pSL2175)pAD::FUS3/2μm/LEU2 67a
 pB45pAD::KAR3(12-515)/2μm/LEU2 62
  • a The two-hybrid constructs created in this study were made by cloning the PCR products into theBamHI or BglII sites of vectors, except for those marked with an asterisk.