Table 8.

Two-hybrid interaction between Bud6p and Ste11p

LexA DNA binding domain fusionGal4p AD fusionRelative β-Gal activity (mean ± SD)a
Bud6p(1-788)Vector0.84 ± 0.08, 0.88 ± 0.05b
Ste11p(22-738)217.06 ± 45.94b
Ste7p(1-515)0.84 ± 0.07
Fus3p(1-353)0.75 ± 0.05
Mkk1p(1-508)0.74 ± 0.07
Mkk2p(1-506)0.98 ± 0.01
Slt2p(1-484)0.72 ± 0.08
Pbs2p(1-668)0.61 ± 0.04
Ste11pVector1.05 ± 0.17
Bud6p(358-768)661.53 ± 300.73
Bud6p(478-788)5.84 ± 0.47
  • a The relative β-Gal activities presented here are normalized to that for Spa2p-vector in Table 4.

  • b See Table 4, footnote b.