Table 4.

Two-hybrid interactions between Spa2p and other polarity protins

LexA DNA binding domain fusionGal4p AD fusionRelative β-Gal activity (mean ± SD)a
Spa2p(1-1466)Vector1.00 ± 0.09, 1.00 ± 0.10b
Pea2p(1-420)17.85 ± 4.58b
Bud6p(275-788)8.30 ± 3.85
Bud6p(358-768)5.37 ± 1.28
Bud6p(478-788)2.35 ± 1.89
Bem1p(1-551)1.37 ± 0.67
Act1p(1-375)1.66 ± 0.12
Bud6p(1-788)Vector0.97 ± 0.18
Bud6p(272-788)Vector2.66 ± 0.18
Bud6p(1-788)Pea2p(1-420)1.98 ± 0.32
Bud6p(272-788)Pea2p(1-420)3.05 ± 0.51
VectorPea2p(1-420)1.10 ± 0.21
Bud6p(275-788)1.06 ± 0.07
Bud6p(358-768)1.06 ± 0.14
  • a The relative β-Gal activities are normalized to that for Spa2p-vector.

  • b Value obtained from analysis of cells grown on plates (see Materials and Methods).