Table 5.

Spa2p interacts with components of MAPK pathways

Reporter strainLexA DNA binding domain fusionGal4p AD fusionRelative β-Gal activity (mean ± SD)a
Wild typeSpa2p(1-1466)Vector1.00 ± 0.09, 1.00 ± 0.10b
Ste11p(22-738)15.21 ± 1.88b
Ste7p(1-515)679.34 ± 30.36b
Fus3p(1-353)1.95 ± 0.36b
Mkk1p(1-508)2,883.54 ± 1,204.52b
Mkk2p(1-506)35.96 ± 8.03
Slt2p(1-484)0.67 ± 0.04
Pbs2p(1-668)0.70 ± 0.02
ste5Δ mutantSpa2p(1-1466)Vector1.00 ± 0.14, 1.00 ± 0.10b
Ste11p(22-738)8.17 ± 1.21b
Ste7p(1-515)117.51 ± 7.11b
Fus3p(1-353)1.91 ± 0.25b
Mkk1p(1-508)963.06 ± 157.88b
Mkk2p(1-506)115.19 ± 12.16
  • a See Table 4, footnote a.

  • b See Table 4, footnote b.