TABLE 3

Plasmids used in this study

NameDescriptionPromoterMarkerSource or reference
pYES2-Q103GFPQ103-GFPGAL1URA324
pRU14DnaJB6bGAL1LEU28
pJE201DnaJB6bGAL1TRP1This study
pJE202DnaJB6bGAL1HIS3This study
pMR360DnaJB6-RFPGAL1LEU28
pNK104JB6-ST onlyGAL1LEU2This study
pNK105JB6-STC onlyGAL1LEU2This study
pNK106ST-RFPGAL1LEU2This study
pNK107STC-RFPGAL1LEU2This study
pRU29DnaJB6ΔSTGAL1LEU2This study
pJE195DnaJB6ΔCTDGAL1LEU2This study
pRU22DnaJB6-D33NGAL1LEU2This study
pJE204DnaJB6D33N-RFPGAL1LEU2This study
pDK58Btn2-RFPGAL1LEU249
pHsp42-CherryHsp42-CherryGAL1LEU229
pJB6-7JJB6 + JB7 J-domainGAL1LEU2This study
pJB6-7CJB6 + JB7 CTDaGAL1LEU2This study
pJB7DnaJB7aGAL1LEU2This study
pJB7-6JJB7a + JB6 J-domainGAL1LEU2This study
pJB7-6CJB7 + JB6 CTDGAL1LEU2This study
pRS315RNQ1-GFPRnq1-GFPRNQ1LEU250
pCUP1-DuDre-Atg8-404RFP-Atg8CUP1TRP151
  • a CTD of DnaJB7 in these constructs lacks residues 227 to 234 and 245 to 309 (Fig. 6A).