TABLE 1

Fission yeast strains used in this studya

StrainGenotypeSource
YM71h leu1-32 ura4-D18Our stock
KO147h leu1-32 ura4-D18 hsk1-89:ura4+Our stock
FY14160h leu1-32 ura4-D18 rif1Δ::ura4+NBRPb
KYP1316h leu1-32 ura4-D18 hsk1-89–Flag3:kanR rif1Δ::ura4+This study
KYP1261h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 Rif1-His6-Flag10:kanROur stock
KYP1262h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 rif1(aa1-1260)-His6-Flag10:kanRThis study
KYP1263h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 rif1(aa1-965)-His6-Flag10:kanRThis study
KYP1264h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 rif1(aa1-442)-His6-Flag10:kanRThis study
KYP1267h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 rif1(aa443-1400)-His6-Flag10:kanRThis study
KYP1311h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 Rif1-His6-Flag10:kanR hsk1-89:ura4+This study
KYP1312h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 rif1(aa1-1260)-His6-Flag10:kanR hsk1-89:ura4+This study
KYP1313h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 rif1(aa1-965)-His6-Flag10:kanR hsk1-89:ura4+This study
KYP1314h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 rif1(aa1-442)-His6-Flag10:kanR hsk1-89:ura4+This study
KYP1315h nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 rif1(aa443-1400)-His6-Flag10:kanR hsk1-89:ura4+Our stock
KYP1328h+ nda3-KM311 leu1-32 ura4-D18 AUR1:aur1r-Adh1-TK-Adh1-ENT1 rif1(aa21-1400Δ)::ura4+This study
KYP1601h nda3-KM311 leu1-32 ura4-D18 rif1(T557A, S1024N, S1063P)-His6-Flag10:kanR AUR1:aur1r-Adh1-TK-Adh1-ENT1This study
KYP1602h+ nda3-KM311 leu1-32 ura4-D18 rif1(Q16R, A352T, S1361P)-His6-Flag10:kanR AUR1:aur1r-Adh1-TK-Adh1-ENT1This study
KYP1603h nda3-KM311 leu1-32 ura4-D18 rif1(R236H)-His6-Flag10:kanR AUR1:aur1r-Adh1-TK-Adh1-ENT1This study
KYP1604h+ nda3-KM311 leu1-32 ura4-D18 rif1(L848S, T865A)-His6-Flag10:kanR AUR1:aur1r-Adh1-TK-Adh1-ENT1This study
KYP1605h nda3-KM311 leu1-32 ura4-D18 rif1(S707P, D731G, I778V, V1265A)-His6-Flag10:kanR AUR1:aur1r-Adh1-TK-Adh1-ENT1This study
KYP1607h+ nda3-KM311 leu1-32 ura4-D18 rif1(E9G, K635R, S1202G)-His6-Flag10:kanR AUR1:aur1r-Adh1-TK-Adh1-ENT1This study
KYP1608h+ nda3-KM311 leu1-32 ura4-D18 rif1L848S-His6-Flag10 AUR1:aur1r-Adh1-TK-Adh1-ENT1This study
KYP1609h+ nda3-KM311 leu1-32 ura4-D18 rif1T865A-His6-Flag10 AUR1:aur1r-Adh1-TK-Adh1-ENT1This study
KYP1610h+ nda3-KM311 leu1-32 ura4-D18 rif1R236H-His6-Flag10 AUR1:aur1r-Adh1-TK-Adh1-ENT1This study
  • a Double colons are used to indicate that the gene of interest is disrupted by insertion of the marker appearing after the double colon. Single colons are used to indicate that the gene of interest is linked to the marker appearing after the single colon.

  • b NBRP, National Bioresource Project.