Table 2.

Effect of overexpression of HAL4 andHAL5 on the intracellular cation concentrations of cells incubated with LiCl and NaCla

StrainPlasmidNo salt, [K+]ib0.1 M LiClb1 M NaClb
[Li+]i[K+]i[Na+]i[K+]i
wtYEp351330 (±30)69 (±3)383 (±6)283 (±10)92 (±5)
YEpHAL4 330 (±20)46 (±4)399 (±3)225 (±9)151 (±8)
YEpHAL5 351 (±12)53 (±4)410 (±5)250 (±50)165 (±7)
ena1-4 YEp351125 (±12)331 (±10)199 (±13)
YEpHAL4 113 (±6)300 (±30)260 (±20)
YEpHAL5 120 (±20)310 (±40)289 (±11)
  • a Strain W303-1A (wt), and itsena1-4 derivative (SKY697) were transformed with either empty plasmid YEp351 or YEp351 carrying HAL4(YEpHAL4) or HAL5 (YEpHAL5). Cells were grown in YPD to exponential phase and treated for 3 h with the indicated concentrations of either LiCl or NaCl.

  • b The intracellular concentrations (millimolar units) of potassium ([K+]i), lithium ([Li+]i), and sodium ([Na+]i) were determined as described in Materials and Methods. Results are the averages of three determinations (± standard deviations).