Table 3.

Nuclear division after release from an HU block

Strain% of cells having two nucleia at:
0 min80 min240 min
YYK7 (sld2-6)865
YHA411 (dpb11-1)5852c
  • a Cells were synchronized with α-factor, released to YPD medium containing 0.2 M HU at 25°C for 90 min, and then transferred to 37°C. After 30 min of incubation, cells were washed and released from the HU block at 37°C. At the indicated time after release from HU, nuclei were observed.

  • b ND, not determined.

  • c Most of these cells have two nuclei with the abnormal morphology described previously (6).