Table 1.

Interaction of different PAP constructs with FIP(20–327) as determined by β-galactosidase activity via two-hybrid analysis

PAP constructNo. of amino acids deletedβ-galactosidase activity (U)a
C-terminal truncations
 GBD-wtPAP0107
 GBD-Δ10PAP31149
 GBD-Δ9PAP43194
 GBD-Δ8PAP551.9
 GBD-Δ6PAP671.1
N-terminal truncations
 wtPAP-GBD093
 Δ14PAP-GBD181.5
  • a Expressed as average units per minute per number of cells from two independent assays.