Table 1.

Features of disease in ΔSH3 c-Abl BMT and Bcr-Abl BMT mice

MouseLatencya (no. of days)Pleural effusionbThymic lymphomacPhenotype of GFP+cellsd
7.28 [Bcr-Abl (b3a2)]17NoNoMac-1+
7.14 [Bcr-Abl (b3a3)]17NoNoMac-1+
ΔSH3 c-Abl
 6.2748YesNoB220+
 6.1672YesNoND
 6.3072YesYesND
 6.2578YesNoB220+
 5.295Yese YesND
 6.2295Yese Yes15% Thy-1.2+/CD4+/CD8+, 85% Thy-1.2+/CD4+/CD8
 6.2398YesNoND
 6.2099YesYesThy-1.2+/CD4+/CD8+
 6.31f 99NoNoB220+/CD43+/HSA+/BP-1+/sIgM
 6.28f 103NoNoB220+/CD43+/HSA+/BP-1+/sIgM
 5.4106YesYesThy-1.2+/CD4+/CD8+
 6.21106YesYesND
 5.3108YesYesThy-1.2+/CD4+/CD8+
 5.6108YesYes60% Thy-1.2+/CD4+/CD8+, 40% Thy-1.2+/CD4+/CD8
  • a Latency is the time after BMT for mice to die or become moribund.

  • b Pleural effusion is 0.5 to 1 ml of unclotted, bloody fluid in the chest cavity, containing erythrocytes and many GFP-positive (GFP+) lymphoblastic cells.

  • c Thymic lymphoma was diagnosed by the presence of a greatly enlarged thymus (0.3 to 0.6 g).

  • d Phenotypes of GFP+ cells were determined by flow cytometry. ND, not determined.

  • e Pleural effusion appeared less bloody, with fewer erythrocytes and WBCs.

  • f Developed rear leg paralysis and elevated peripheral WBCs with >50% GFP+ lymphoblastic cells. No tumors, pleural effusion, or lung hemorrhages were seen.