Table 3.

Eya domain is not sufficient for nuclear translocationa

Six proteinDistribution (%)
Eya1EDEya2EDEya3ED
NucleusCytoplasmNucleusCytoplasmNucleusCytoplasm
None4.695.41.398.76.693.4
Six4NDb ND2.897.2NDND
Six58.491.6NDND6.393.7
  • a The relative amounts of nuclear and cytoplasmic Eya domain proteins from COS7 cells coexpressed with Six4 or Six5 were calculated as described for Table 1. The amount of DNA used for transfections was 10 μg each. Three independent sets of experiments yielded essentially the same results.

  • b ND, not determined.