Table 4.

Interaction between various domains of Eya2 and Six4SDHDa

Eya2 subdomain proteinInteraction with Six4SDHD
Eya2++
Eya2ED
Eya2ΔED
Eya2NΔ1++
Eya2NΔ2++
Eya2NΔ3+
  • a Yeast strain EGY48 was cotransformed with pJG4-5 constructs expressing various Eya2 subdomain proteins and pEG202Six4SDHD. An interaction was scored as strongly positive (++) or positive (+) if the transformant turned blue on X-Gal indicator plates containing galactose and negative (−) if it did not. The regions contained in pJG4-5 Eya2 constructs are shown in Fig. 1E.