Table 2.

Effects of not1 truncations onADH2 expressiona

NOT1 plasmidLexA plasmidADH II activity (mU/mg; mean ± SEM)b
NOT1(396–2108)None2,900 ± 450
NOT1(1319–2108)None910 ± 150
LexA-NOT1(1–2108)2,300 ± 150
LexA-NOT1(1–1152)1,800 ± 120
LexA-NOT1(1–667)830 ± 100
NoneLexA-NOT1(1–2108)2,200 ± 320
LexA-NOT1(1490–2108)1,000 ± 230
  • a ADH II assays were conducted after growth of strain 1422–21 on YEP medium containing 3% ethanol. ADH II activities in a strain carrying an integrated full-lengthNOT1 gene is generally 2,500 to 3,000 mU/mg. LexA plasmids contain NOT1 sequences fused to LexA-202(7). NOT1 plasmids were pRS426 derivatives.

  • b Average of at least three separate transformants.