Table 1.

Role of regions I, II, and III in replication ofars2004

PlasmidRegionColony formationaColony sizebMitotic stabilityc (%)
IIIIII
2004++++Large2.2 ± 0.8
2004Δ940NT
2004ΔI+++Medium8.4 ± 1.7
2004ΔII+++Large3.2 ± 1.8
2004ΔIII+++Medium5.1 ± 2.0
2004ΔIΔII++/−Small14.0 ± 1.6
2004ΔIΔIII+NT
2004ΔIIΔIII++Medium8.5 ± 3.2
  • a The number of transformants was counted after incubation for 3 days at 30°C. +, efficiency similar to that ofars2004; +/−, about 20% of the efficiency ofars2004; −, no transformant formed.

  • b Transformants formed after a 3-day incubation were classified into three groups based on colony size.

  • c The mitotic loss rate of plasmid per generation was determined as described in Materials and Methods. NT, not tested.