Table 1.

Yeast strains and plasmids used in this study

StrainRelevant genotypeaPlasmid description (name; source or reference)
YAS1070MATaTRP1pPAB1 URA3 LEU2 CEN (pAS580; this study)
YAS1071MATα lys2pPAB1 URA3 LEU2 CEN(pAS580; this study)
YAS1915MATapPAB1 TRP1 CEN (pAS80)
YAS2266MATapPAB1 URA3 CEN(pAS77; 35)
pTRP1 CEN (pUN15;11)
YAS2267MATapPAB1 URA3 CEN (pAS77)
pSPB8 TRP1 CEN (pAS579; this study)
YAS2268MATa spb8-1pPAB1 URA3 CEN(pAS77)
pTRP1 CEN (pUN15)
YAS2269MATaspb8-1pPAB1 URA3 CEN (pAS77)
pSPB8 TRP1 CEN (pAS579)
YAS2270MATα spb8-2 lys2pPAB1 URA3 CEN (pAS77)
pTRP1 CEN (pUN15)
YAS2271MATα spb8-2 lys2pPAB1 URA3 CEN (pAS77)
pSPB8 TRP1 CEN (pAS579)
YAS2272MATapPAB1 TRP1 CEN (pAS80;35)
pGAL1 MFA2pG URA3 CEN (pRP485;9)
YAS2273MATα spb8-2 lys2pPAB1 TRP1 CEN (pAS80)
pGAL1 MFA2pG URA3 CEN (pRP485)
YAS2274MATapPAB1 TRP1 CEN (pAS80)
pGAL1 B55TPGK1pG URA3 CEN (pRP602;27)
YAS2275MATα spb8-2 lys2pPAB1 TRP1 CEN (pAS80)
pGAL1 B55TPGK1pG URA3 CEN (pRP602)
YAS2276MATapPAB1 TRP1 CEN(pAS80)
pGAL1 B55TPGK1NSpG URA3 CEN (pRP611; 26)
YAS2277MATα spb8-2 lys2pPAB1 TRP1 CEN (pAS80)
pGAL1B55TPGK1NSpG URA3 CEN (pRP611)
YAS2278MATα spb8-2 lys2pPAB1 TRP1 CEN (pAS80)
YAS2279MATα spb8-2 lys2pGAL1 MFA2pG URA3 CEN(pRP485)
YAS2280MATα ADE2 lys2pPAB1 URA3 TRP1 CEN(pAS521; this study)
YAS2281MATapPAB1 URA3 TRP1 CEN (pAS521; this study)
  • a All strains are PAB1::HIS3 ade2-1 his3-11 leu2-3,112 trp1-1 ura3-1 can 1-100unless otherwise stated.