Table 2.

Sequences of mutagenic NS5A oligonucleotides

ConstructMutagenic primeraMutation(s)
NS5A 1b-25′ GACTCCCCAGATGTTGACCTCATCA2224V
NS5A 1b-5b
NS5A 1b-45′ TTGTCTGCGCTTTCCTTGAAGGCAGCATGCACTGGCCGTCACGACP2209L, T2214A, T2217G
  • a Underlined codons correspond to the indicated amino acid mutations.

  • b See Materials and Methods for details on the construction of this construct.