Table 1.

Antisense oligonucleotides against p21 mRNA block cell cycle exit upon myogenic differentiation

AdditionaDNA content (%)b
G0/G1SG2/M
None622711
p21-AS384517
Control67258
  • a C2C12 cells were cultured in differentiation medium in the presence or absence (none) of 0.5 μM phosphorothioate antisense oligonucleotides to p21 (p21-AS) or control oligonucleotides for 24 h.

  • b Determined by fluorescence-activated cell sorting analysis.