Table 1.

Yeast strains and plasmids

Strain or plasmidGenotypeSource or reference
Strains
 YJO235 MAT a CDC7-myc9leu2-3,112 ura3-52 trp1-289 bar1::LEU2 This study
 YJL310 MAT a leu2-3,112 ura3-52 trp1-289 bar1::LEU2 This study
 YJO280 MAT a CDC7-myc9leu2-3,112 ura3-52 trp1-289 bar1::LEU2 dbf4-1 This study
 GOY244 MAT a ura3 trp1 can cdc16-1 pep4 prb1 + pYQ117This study
 GOY263 MAT a YJO235 + pYQ117This study
 GOY264 MAT a YJO235 + pYQ118This study
 237 MAT a bar1 his6 ura3 trp1 leu2 cyh2 can1 ade1 This study
 271 MAT a his6 bar1 trp1-289 can1 sap3 cdc16-1 This study
Plasmids
 pYQ117pYES2-His-HAThis study
 pYQ118pYES2-His-HA-DBF4This study
 pJO36pRS306-CDC7-myc9 This study
 pEG.MCM2GST-MCM2 44
 pEG.MCM3GST-MCM3 44
 pEG.MCM4GST-MCM4 44
 pEG.MCM5GST-MCM5 44
 pEG.MCM6GST-MCM6 44
 pEG.MCM7GST-MCM7 44