Table 1.

Sequences of oligonucleotides used for DNA binding and construction of pGbe-lacZ derivatives

Name Sequencea
A15′-tcgagGGTGGCAGGTGCCATTg
A25′-tcgagAGATCTACGCAGGTGGTTCTTGTg
B15′-tcgagGGTGGCACGTGCCATTg
B25′-tcgagTTCTAGCACGTGTCACCAGg
B35′-tcgagGGGTCCACGTGAGCTTg
Hairy5′-tcgagAGCCGGCACGCGACAGGg
N box5′-GATCACGCCACGAGCCACAAGGATTG
  • a Only one strand is shown. The E-box elements are in bold, and E boxes along with three flanking bases are underlined. Additional bases used to create restriction enzyme sites at the termini are in lowercase letters. Sequences of the N-box oligonucleotides are the same as those used previously (65), and the Hairy oligonucleotide sequence is derived from the regulatory region of the achaete gene (49, 67).