TABLE 1

Classification of sequences obtained by deep sequencinga

Sequence typebNo. of reads
Library 1Library 2
rTERT-RNA 1rTERT-RNA 2DMSO-RNA 1Nocodazole-RNA 1
A1,159,55514,77631,3558,804
B39,340,94943,335,02593,651,64354,932,629
C0000
D251,67217,945118,3204,287,182
Other141,370,21460,833,72968,867,660102,515,449
Total reads182,122,390104,201,475162,668,978161,744,064
  • a The RNA products of the IP-RdRP assay were analyzed by deep sequencing. The obtained sequences (>6 nt) were mapped to the template sequence and classified into five categories (type A to D and “other”), as indicated. The number of total reads indicates the number of sequences used for mapping.

  • b Type A, completely identical to template RNA; type B, completely identical to a part of the sequence of template RNA; type C, completely complementary to template RNA; type D, completely complementary to a part of the sequence of template RNA.