Table 1.

Primers designed for quantitative real-time RT-PCR of genes and ChIP qPCR

Analysis and genePrimer
OrientationbSequence (5′-3′)c
qRT-PCRa
    mG9aLFCAA GGA TGG CGA GGT TTA CTG
RTGC GTG GGA ACC GTA GAT CT
    mSuv39h1FCGG ATC ACC GTG GAG AAT G
RCAC TCA CAG CCA ACA GCT ACC T
    hSuv39H1FATC CGC GAA CAG GAA TAT TAC C
RGAG GAT ACG CAC ACA CTT GAG ATT
    mSuv39h2FCTG ATG AGT TCA CAG TGG ATG CA
RTTT GGG TCA CAA CTA TGA TTC ACA
    hSuv39H2FCCC AAA TCT TCA GGT GTT CAA TG
RGGT TCT TGT GGA AAA CAA TGC TAT T
    mESETFTCA CGG AAA CAA GTA GCC AAG A
RGCC AAA GGT GAC CGA TAT GTC T
    mGLP/EuMFTTC AGG CGG GTG CCA ATA T
RCTG CGT CCT TCG GAT CCA
TaqMan assay
    mSuv39h1Mm00468952_m1
    hSuv39H1Hs00162471_m1
    mSuv39h2Mm00469689_m1
    mSuv39H2Hs00226596_m1
ChIP: mSP-AFCAC TTG GGA GTT TGC CTT CTG
RAGG AAG CAG CCA TCG TGT GTA
  • a The primers were designed to determine specific expression using SYBR green qRT-PCR.

  • b F, forward; R, reverse.

  • c For the four TaqMan assay primers, the Applied Biosystems reference numbers are given.