Table 1.

Yeast strains used in this study

StrainGenotypeReference (plasmid reference[s]) or source
YF336 (BY4741)mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ064
YF534mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 rad6Δ::KanMXSaccharomyces Genome Deletion Project
YF688mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 pho23Δ::KanMXSaccharomyces Genome Deletion Project
YF1557mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 MUD2-TAP::HIS3Open Biosystems TAP-Fusion Library
YF1703mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 rtr1Δ::KanMXSaccharomyces Genome Deletion Project
YF1495mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 yng1Δ::KanMXSaccharomyces Genome Deletion Project
YF1471matα ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 can1-100 nab3-1116
YSB2086mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 {pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2094mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 {pJC643 [nrd1(1-164Δ)-HA2 LEU2 CEN]}60 (16)
YSB2152mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX {pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2153mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX {pJC643 [nrd1(1-164Δ)-HA2 LEU2 CEN]}This study (16)
YSB2253mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 set1Δ::HISMX6T. Kim
YSB2256mata ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2-801 ade2-101 hht1-hhf1::pWZ405-F2F9-LEU2 hht2-hhf2::pWZ403-F4F10-HIS3 {pJH18-H3-H4-WT [HHT2(histone H3) HHF2(histone H4) TRP1 CEN]}T. Kim (5)
YSB2257mata ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2-801 ade2-101 hht1-hhf1::pWZ405-F2F9-LEU2 hht2-hhf2::pWZ403-F4F10-HIS3 {pRS314-H3(K4R)/H4[hht2(K4R) (histone H3) HHF2(histone H4) TRP1 CEN]}T. Kim (plasmid, M. Keogh)
YSB2325mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 MUD2-TAP::HIS3 rad6Δ::KanMXThis study
YSB2409mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 pho23Δ::KanMX{pJC643 [nrd1(1-164Δ)-HA2 LEU2 CEN]}This study (16)
YSB2412mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 pho23Δ::KanMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2415mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 rad6Δ::KanMX{pJC643 [nrd1(1-164Δ)-HA2 LEU2 CEN]}This study (16)
YSB2420mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 pho23Δ::KanMX set1Δ::TRP1{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2421mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 pho23Δ::KanMX set1Δ::TRP1{pJC643 [nrd1(1-164Δ)-HA2 LEU2 CEN]}This study (16)
YSB2488mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 rtr1Δ::KanMX {pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2489mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 yng1Δ::KanMX {pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2491mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX rtr1Δ::NATMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2492mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 pho23Δ::KanMX yng1Δ::NATMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2502mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN pRS416-SET1(1-1080) (Flag-SET1 URA3 CEN)]}This study (18, 61)
YSB2503mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN pRS416-SET1RRM) [Flag-SET1(230-335Δ) URA3 CEN]}This study (18, 61)
YSB2504mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN] pRS416-SET1(780-1080) [Flag-SET1(1-779Δ) URA3 CEN]}This study (18, 61)
YSB2505mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN pRS416-set1(H422A) [Flag-set1(H422A) URA3 CEN]}This study (61)
YSB2506mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN pRS416-set1(H1017K) [Flag-set1(H1017K) URA3 CEN]}This study (61)
YSB2507mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pJC643 [nrd1(1-164Δ)-HA2 LEU2 CEN] pRS416-SET1(1-1080) (Flag-SET1 URA3 CEN)}This study (16, 18)
YSB2508mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pJC643 [nrd1(1-164Δ)-HA2 LEU CEN] pRS416-SET1RRM) [Flag-SET1(230-335Δ) URA3 CEN]}This study (16, 18)
YSB2509mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pJC643 [nrd1(1-164Δ)-HA2 LEU2 CEN] pRS416-SET1(780-1080) [Flag-SET1(1-779Δ) URA3 CEN]}This study (16, 18)
YSB2510mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pJC643 [nrd1(1-164Δ)-HA2 LEU2 CEN] pRS416-set1(H422A) [Flag-set1(H422A) URA3 CEN]}This study (16)
YSB2511mata ura3-52 leu2-3,112 trp1-1 his3-11,15 lys2Δ2 ade2-1 met2Δ1 can1-100 nrd1Δ::HIS3 set1Δ::KanMX{pJC643 [nrd1(1-164Δ)-HA2 LEU2 CEN] pRS416-set1(H1017K) [Flag-set1(H1017K) URA3 CEN]}This study (16)
YSB2559matα ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 can1-100 nab3-11 set1Δ::TRP1This study
YSB2397mata ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2-128δ rrp6Δ::KanMXThis study
YSB2398mata ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2-128δ set1Δ::HISMX6 rrp6Δ::KanMXThis study
YSB2582matα ura3-52 leu2-3,112 his3Δ200 set1Δ::KanMX nrd1Δ::HIS3{pRS415-nrd1(151-214) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2583matα ura3-52 leu2-3,112 his3Δ200 set1Δ::KanMX nrd1Δ::HIS3{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN] rpb2-10[rpb2-10(P1018S) URA3]}This study (45, 61)
YSB2574matα ura3-52 leu2-3,112 his3Δ200 pho23Δ::NATMX nrd1Δ::KanMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN]}This study (61)
YSB2595matα ura3-52 leu2-3,112 his3Δ200 pho23Δ::NATMX nrd1Δ::KanMX{pRS415-nrd1(151-214Δ) [nrd1(151-214Δ)-HA2 LEU2 CEN rpb2-10 [rpb2-10(P1018S) URA3]}This study (45, 61)
YSB2064mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 nrd16-150Δ60
YSB2665mata ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 nrd16-150Δ set1Δ::KanMXThis study