Table 1.

Primer sequences used in this study

Primer usePrimer sequencesa
Genotyping
    MTBFwd, 5′-TGCCGCCATTATTACGACAAGC-3′; Rev, 5′-ACCGTACTCGTCAATTCCAAGGG-3′
    Gata-3+/TgFwd, 5′-ACGTCTCACTCTCGAGGCAGCATG-3′; Rev, 5′-GAAGTCCTCCAGCGCGTCATGCAC-3′
    Gata-3+/nlslacZFwd, 5′-CAGGAGTCCGCGGACCTCC-3′; Rev, 5′-CGTTGAGGACCGCGGGGTG-3′; Rev, 5′-CCAAGCTTGGACTCAAAAAAC-3′
    MMTV-PyMTFwd, 5′-CTCTAGAGGATCTTTGTGAAGG-3′; Rev, 5′-GGACAAACCACAACTAGAATGC-3′
Gene expression analysis
    β-CaseinFwd, 5′-AAAGGACTTGACAGCCATGAA-3′; Rev, 5′-TAGCCTGGAGCACATCCTCT-3′
    Gata-3Fwd, 5′-AGCCACATCTCTCCCTTCAG-3′; Rev, 5′-AGGGCTCTGCCTCTCTAACC-3′
    Caspase-14Fwd, 5′-GATGAGGTTGCTGTGCTCAA-3′; Rev, 5′-GAACACATCCGTCAGGGTCT-3′
    GAPDHFwd, 5′-GTATTGGGCGCCTGGTCACC-3′; Rev, 5′-CGCTCCTGGAAGATGGTGATGG-3′
    18S rRNAFwd, 5′-GTAACCCGTTGAACCCCATT-3′; Rev, 5′-CCATCCAATCGGTAGTAGCG-3′
Chromatin immunoprecipitation
    ControlFwd, 5′-AGGCATCTCTTTGAGATGGCT-3′; Rev, 5′-TGGGTCATTCACCAGCTTCT-3′
    −484Fwd, 5′-CTGTCTGTGCAGCCAGAGAA-3′; Rev, 5′-AGCAGGGCAAGTGACCCATGTG-3′
    −3060Fwd, 5′-CCTTATAGGGTTGAATGCCAA-3′; Rev, 5′-TGGCCTAGGCAGAGAGTCT-3′
    −3772Fwd, 5′-GACTGGCAGGTAGGGAAGGTGAG-3′; Rev, 5′-ATAGTGAAACCAAAGGCGAAC-3′
    −4392Fwd, 5′-ATCTCACACTTTGGGAGGCCGAGTG-3′; Rev, 5′-ACTCGGTTCGATAGCAACAGCT-3′
Plasmid construction
    Gata-3 Tg (pTet-On-Gata-3)Fwd, 5′-GGAAGATCTCACCATGGACTACAAAGACGATGACGATAAAGAGGTGACTGCGGACCAGCCGCGC-3′; Rev, 5′-GGAAGATCTCTAACCCATGGCGGTGACCAT-3′
    pFU-TA-GFP Gata-3Fwd, 5′-TGCGAATTCATGGAGGTGACGGCGGACCAGCCGCGC-3′; Rev, 5′-CCGAGATCTACCCATGGCGGTGACCATGCTGGA-3′
    pFU-TA-GFP caspase-14Fwd, 5′-CGCGAATTCTCACCATGAGCAATCCGCGGTCTT-3′; Rev, 5′-CCGGGATCCCTGCAGATACAGCCGTTTCCGGA-3
  • a Fwd, forward; Rev, reverse.