Table 2.

Limiting dilution analysis of the repopulating frequency of CD29hi CD24+ MaSC-enriched cells from control or MMTV-cre; Gata-3f/f mammary glandsa

RecipientNo. of CD29hi CD24+ cells injected per mammary fat padNo. of positive outgrowthsb
Gata-3+/fMMTV-cre; Gata-3f/f
Rag1−/−754/90/9
1501/91/9
2508/112/10
5007/83/8
    Repopulating frequency (95% confidence interval)1/254 (1/406–1/159)1/1,232 (1/2,737–1/554)
    P value0.0003
NOD-SCID2502/21/2
5003/40/4
    Repopulating frequency (95% confidence interval)1/244 (1/670–1/87)1/2,373 (1/17,594–1/320)
    P value0.016
  • a Limiting dilution analysis of the repopulating frequency of CD29hi CD24+ cells (double sorted) from the mammary glands of Gata-3+/f or MMTV-cre;Gata-3f/f mice. Cells were injected into the cleared mammary fat pads of 3-week-old Rag1−/− or NOD-SCID immunocompromised recipients and fat pads collected 8 weeks posttransplantation. Data are pooled from three (Rag1−/−) or two (NOD-SCID) independent experiments for each cell type.

  • b Shown as number of outgrowths per number of injected fat pads.