Table 2.

Ch16 minichromosome loss rates

Genetic backgroundLoss rate% division
Wta12/9,9470.12
wpl1Δ14/4,9310.28
eso1Δ wpl1Δ16/5,3240.30
eso1Δ psm3K105NNDb∼100
eso1Δ psm3K105N wpl1Δ0/2,931<0.03
eso1Δ psm3K106NND∼100
eso1Δ psm3K106N wpl1Δ5/6,0540.08
eso1Δ psm3K105NK106N50/2,4742.02c
eso1Δ psm3K105NK106N wpl1Δ4/5,6940.07
psm3K105NK106N25/7,3270.34
psm3K105NK106N wpl1Δ17/6,3700.27
psm3K105R14/5,8180.24
psm3K105R wpl1Δ19/6,2500.30
psm3K106R55/7,7720.71c
psm3K106R wpl1Δ5/6,9110.07
psm3K105RK106R66/8,3710.79c
psm3K105RK106R wpl1Δ10/6,3920.16
  • a Wt, wild type.

  • b ND, not determined, since Ch16 could not be maintained in this genetic background. The loss rate is therefore estimated as 100%.

  • c Statistically significant compared to wt (P value < 0.0001 by the chi-square test).