Table 1

Yeast strains used in this study

StrainGenotypeSource or reference
W303MATα ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100R. Rothstein
BY4741MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ043
MHY4201MATα ulp1Δ::kanMX4 his3Δ0 leu2Δ0 ura3Δ0 (YCplac22-ulp1ts-NAT)28
MHY4479MATα ulp1Δ::kanMX4 his3Δ0 leu2Δ0 ura3Δ0 (pRS415-ulp1-N*-GFP)This study
MHY4480MATα ulp1Δ::kanMX4 his3Δ0 leu2Δ0 ura3Δ0 (pRS415-ulp1-C*-GFP)This study
MHY4566MATa yra1-2::his5+ ade2-1 ura3-52 his3-11 trp1-11 leu2-3,112 can1-100 bar1Δ24
MHY4709MATα ulp1Δ::LEU2 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 (YCplac22-ulp1ts)This study
MHY4806MATα ulp1Δ::LEU2 yra1-2::his5+ ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 (YCplac22-ulp1ts)This study
MHY5341MATα ulp1Δ::LEU2 yra1-2::his5+ kanMX6::GAL1-H6-SMT3 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 (YCplac22-ulp1ts)This study
MHY5343MATα ulp1Δ::LEU2 yra1-2::his5+ kanMX6::GAL1-SMT3 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 (YCplac22-ulp1ts)This study
MHY5809MATα ubc9Δ::TRP1 ubc9ts::LEU2 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100This study
MHY5816MATα ulp2Δ::HIS3 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100This study
MHY5891MATα scs2Δ::kanMX4 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100This study
MHY5893MATα scs2Δ::kanMX4 ulp1Δ::LEU2 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 (YCplac22-ulp1ts)This study
MHY5904MATα SCS2-V5::kanMX6 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100This study
MHY5910MATα SCS2-V5::kanMX6 ulp1Δ::LEU2 yra1-2::his5+ ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 (YCplac22-ulp1ts)This study
MHY5941MATα SCS2-V5::kanMX6 ulp1Δ::LEU2 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 (YCplac22-ulp1ts)This study
MHY6000MATα opi1Δ::kanMX4 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 can1-100This study
MHY6006MATα opi1Δ::kanMX4 ulp1Δ::LEU2 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 (YCplac22-ulp1ts)This study
MHY6147MATα ulp2Δ::HIS3 scs2Δ::kanMX4 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112This study
MHY6259MATα scs2Δ::kanMX4 ubc9Δ::TRP1 ubc9ts-LEU2::leu2-3,112 ade2-1 ura3-1 his3-11,15 trp1-1 can1-100This study
MHY6405MATα opi1Δ::kanMX4 ubc9Δ::TRP1 ubc9ts-LEU2::leu2-3,112 ade2-1 ura3-1 his3-11,15 trp1-1 can1-100This study
MHY6595MATα ulp1Δ::kanMX4 his3Δ0 leu2Δ0 ura3Δ0 (pRS415-ulp1-N*-3N-GFP)This study
MHY6624MATα opi1Δ::kanMX4 ulp2Δ::HIS3 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100This study
MHY6770MATα ulp1Δ::kanMX4 his3Δ0 leu2Δ0 ura3Δ0 (pRS415-ULP1-GFP)This study
MHY6772MATα ulp1Δ::kanMX4 his3Δ0 leu2Δ0 ura3Δ0 (pRS415-ulp1-N*-3C-GFP)This study
MHY6774MATα ulp1Δ::kanMX4 his3Δ0 leu2Δ0 ura3Δ0 (pRS415-ulp1-Q272R,N332S-GFP)This study
MHY6776MATα ulp1Δ::kanMX4 his3Δ0 leu2Δ0 ura3Δ0 (pRS415-ulp1-K352E-GFP)This study