Table 2

Plasmids used in this study

PlasmidDescriptionSource or reference
16-9-9YCplac22-ulp1ts(333)29
18-6-3pFA6a-kanMX6-PGAL133
2-8-8pRS31642
4-1-4YCplac3312
43-1-2pFA6a-6√óGly-V5-kanMX611
47-6-2pRS316-SCS2This study
47-7-1pRS316-scs2-K180R,K181RThis study
48-5-3YCplac33-HA-scs2-K181RThis study
48-5-7YCplac33-HA-scs2-K180R,K181RThis study
48-6-6YCplac33-HA-scs2-K180RThis study
49-4-1pRS415-ulp1-N*-3N-GFPThis study
54-3-6pRS316-SMT3(GG)This study
56-2-1pRS415-ulp1-N*-3C-GFPThis study
56-4-5pRS415-ulp1-Q272R,N332S-GFPThis study
56-6-9pRS415-ULP1-GFPThis study
56-7-5pRS415-ulp1-K352E-GFPThis study
BOK32pRS415-ulp1-N*-GFPThis study
BOK35pRS415-ulp1-C*-GFPThis study
BOK58YCplac22-ulp1ts-NAT (NAT marker inserted into AflII site of ulp1ts)This study
pKY166YCplac33-HA-SCS223
pMR1036INO1-CYC-lacZ reporter7
60-4-3pRS426-INO146