Table 3

Proteins identified as sumoylated substrates by the presence of a ≥3-fold-higher number of peptides

Protein (predicted mass in kDa)No. of peptides per sample expressing:Group(s)aFunctionb
Untagged Smt3 (S)His6-Smt3 (H)
Cdc3 (60)062Component of the septin ring
Cdc48 (92)061ATPase with homology to mammalian p97
Gpd1 (43)031NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis
Hsp104 (102)061, 2Heat shock protein that cooperates with Ydj1 and Ssa1 to refold and reactivate denatured, aggregated proteins
Lys1 (41)031Saccharopine dehydrogenase; catalyzes the conversion of saccharopine to l-lysine
Pol30 (29)051Proliferating cell nuclear antigen; functions as the sliding clamp for DNA polymerase delta
Pgk1 (45)8341, 23-Phosphoglycerate kinase; catalyzes the transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP
Rps0a (28)031Protein component of the small (40S) ribosomal subunit
Rps0b (28)031Protein component of the small (40S) ribosomal subunit
Rps3 (27)031Protein component of the small (40S) ribosomal subunit
Rsc8 (63)042Component of the RSC complex
Scs2 (27)071, 2Integral ER membrane protein that regulates phospholipid metabolism
Tfg1 (82)042TFIIF largest subunit; involved in both transcription initiation and elongation of RNA polymerase II
Tup1 (78)082General repressor of transcription
Yel007w (73)032Putative protein with sequence similarity to Schizosaccharomyces pombe gti1+ (gluconate transport inducer 1)
Zuo1 (49)031Cytosolic ribosome-associated chaperone
  • a Group 1 consists of proteins of 35 to 60 kDa. Group 2 consists of proteins of 60 to 150 kDa.

  • b RSC complex, chromatin structure remodeling complex; TFIIF, transcription factor II.