Table 2

Resection rates for mutants

Strain genotypeRate of DNA resection (kb/h)a
Wild type4.0 ± 0.7b
fun30Δ1.2 ± 0.1c
sgs1Δ3.0 ± 0.2b
sgs1Δ fun30Δ1.8 ± 0.7b
exo1Δ2.1 ± 0.4c
fun30Δ exo1Δ1.2 ± 0.2b
H2A-S129A6.8
fun30Δ H2A-S129A2.4
  • a 5′-to-3′ resection rates from an HO-induced unrepaired DSB in strain JKM179 and its derivatives were measured as described in the legend to Fig. 3 and in Materials and Methods. Resection rates were calculated from qPCR values as described in the text.

  • b Average of three time courses (± standard deviation).

  • c Average of two time courses (± range).