Table 3

CDK interactions of cyclin K1, K2, T1, and L1 identified by IP-MSa

Bait proteinCell lineNo. of unique peptides/total no. of peptides (X!Tandem score)
CyclinCDK:
1213 (Cdc2L5)911
Cyclin L1-3×FLAGHEK293A4/4 (−24.8)0001/1 (−1.2)
HEK293A3/3 (−18.7)0009/14 (−69.4)
3×FLAG-cyclin T1HEK293A5/5 (−36.7)005/6 (−33.9)0
Endogenous cyclin T1HEK2931/1 (−1.6)0014/18 (−114.3)0
HeLaS311/32 (−98.9)0016/25 (−136.3)0
Cyclin K2-3×FLAGHEK293A12/17 (−102.9)14/16 (−112.8)8/11 (−83.8)00
HEK293A4/4 (−29.5)8/9 (−66.6)1/2 (−10.5)1/2 (−7.9)
3×FLAG-cyclin K1HEK293A16/60 (−157.5)8/14 (−66.3)3/5 (−23.1)00
HEK293A17/92 (−187.9)6/12 (−44.8)1/6 (−17.2)2/6 (−17.1)0
  • a Endogenous and overexpressed epitope-tagged cyclin complexes were isolated by immunoprecipitation and analyzed by MS for CDKs. Cyclin L1- or K2-3×FLAG, N-terminally FLAG-tagged cyclin L1 or K2; 3×FLAG-cyclin T1 or K1, C-terminally FLAG-tagged cyclin T1 or K1.