Table 1

Primers used in this study

PrimerDescriptionaSequence
G1P-FGPD1 promoter FCTGACTCGAGAGCCCGAAAGAGTTATCGTTACTC
G1-FGPD1 ORF FTCGAGGATCCATGTCTGCTGCTGCTGATAG
G1-RGPD1 ORF RCTGAGCGGCCGCCATGGTGATGGTGATGGTGATCTTCATGTAGATCTAATTCTTC
G1SA-FGPD1 S24A FGGTAGAAAGAGAAGTTCCGCTTCTGTTTCTTTGAAGGCTG
G1SA-RGPD1 S24A RCAGCCTTCAAAGAAACAGAAGCGGAACTTCTCTTTCTACC
G1SSEE-FGPD1 S24E/S27E FGGTAGAAAGAGAAGTTCCGAATCTGTTGAATTGAAGGCTGCCGAAAAG
G1SSEE-RGPD1 S24E/S27E RCTTTTCGGCAGCCTTCAATTCAACAGATTCGGAACTTCTCTTTCTACC
G2P-FGPD2 promoter FCTGACTCGAGCAAAAACGACATATCTATTATAGTG
G2-RGPD2 ORF RCTGAGCGGCCGCCATGGTGATGGTGATGGTGTTCGTCATCGATGTCTAGCTCTTC
G2SA-FGPD2 S72A FCCTATCAGAAGATCGGACGCAGCCGTGTCAATTGTACAT
G2SA-RGPD2 S72A RCTGAGCGGCCGCCATGGTGATGGTGATGGTGTTCGTCATCGATGTCTAGCTCTTC
G2+3SA-FGPD2 S75A FTCGGACTCTGCCGTGGCAATTGTACATTTGAAAC
G2+3SA-RGPD2 S75A RGTTTCAAATGTACAATTGCCACGGCAGAGTCCGA
G2PG1-RGPD2 promoter-GPD1 RCCTTCGCTCCCCTTCCTTATCAATGTCTGCTGCTGCTGATAG
G2PG1-FGPD2 promoter-GPD1 FCTATCAGCAGCAGCAGACATTGATAAGGAAGGGGAGCGAAGG
G2KO-Fgpd2Δ::HIS3 FTTCTCTTTCCCTTTCCTTTTCCTTCGCTCCCCTTCCTTATCAATGTCTACCGGCAGATCCGCTAG
G2KO-Rgpd2Δ::HIS3 RAGGCAACAGGAAAGATCAGAGGGGGAGGGGGGGGGAGAGTGTCTA GAATTCGAGCTCGTTTAAAC
G2KO-AGPD2 5′ UTR (−370)CTCTGCCATTGTTATATTACGCTTT
G2KO-DGPD2 3′ UTR (+334)GAGAATTACTGCAGTGAAAAAGCTC
HIS3-BHIS3 RTTGCGCTCCTGAACAGTTTG
HIS3-CHIS3 FATTGGATGAGGCGCTATCACG
SNF1-FSNF1 cat domain FCAGTGGATCCAATCCCAAGTCGTCCTTAGC
SNF1-RSNF1 cat domain RGTGAGCGGCCGCTCAAAGTAGATATTCTGGCAGGTC
  • a F, forward; R, reverse; UTR, untranslated region.