Table 2

Yeast plasmids used in this study

PlasmidDescriptionReference or source
pBG1805-Gpd12μm URA3 PGAL1-GPD1-6×His-HA-ZZ32
pBG1805-Gpd1-S24ApBG1805-Gpd1 S24A mutantThis study
pBG1805-Gpd1-SSEEpBG1805-Gpd1 S24E,S27E mutantThis study
pBG1805-Gpd22μm URA PGAL1-Gpd2-6×His-HA-ZZ32
pBG1805-Gpd2-S72ApBG1805-Gpd2 S72A mutantThis study
pBG1805-Gpd2-SSEEpBG1805-Gpd2 S72E,S75E mutantThis study
p415-GFPCEN LEU2 GFP TCYC1M. Hochstrasser
p415-Gpd1CEN LEU2 GPD1-6×His-GFP TCYC1This study
p415-Gpd1-S24Ap415-Gpd1 S24A mutantThis study
p415-Gpd1-SSEEp415-Gpd1 S24E,S27E mutantThis study
p415-Gpd2CEN LEU2 GPD2-6×His-GFP TCYC1This study
p415-Gpd2-S72Ap415-Gpd2 S72A mutantThis study
p415-Gpd2-SSEEp415-Gpd2 S72E, S75E mutantThis study
p415-G2P-Gpd1CEN LEU2 PGPD2-GPD1-6×His-GFP TCYC1This study
p415-G2P-Gpd1-SAp415-G2P-Gpd1 S72A mutantThis study
p415-G2P-Gpd1-SSEEp415-G2P-Gpd1 S72E, S75E mutantThis study
pRS315-Ypk111A-mycCEN LEU2 YPK1-11A-myc57
pRS316-Snf1CEN URA3 SNF1-3×HAaM. Schmidt
pRS315-Mig1CEN LEU2 MIG1-3×HAM. Schmidt
  • a HA, hemagglutinin.