TABLE 1

Identification of LRRK2 WD40 interactors

Accession no.aProtein nameGeneExperiment(s)bMass (kDa)Coverage (%)cNo. of WD40 repeatsdSourcee
P60711 (P60710)Actin, betaActbMS, WB, IP4246.017LS1, total brain
P85515Alpha-centractinActr1aMS, WB4316.04LS1 fraction
P39069Adenylate kinase 1Ak1MS2214.02LS1 fraction
Q9DBG3Adaptor-related protein complex 2, beta 1 subunitAp2b1MS1054.04Total brain
Q08163Adenylyl cyclase-associated protein 1Cap1MS526.02LS1 fraction
P84079ADP-ribosylation factor 1Arf1MS2115.02LS1 fraction
Q5XI73Rho GDP dissociation inhibitor (GDI) alphaArhgdiaMS2333.04LS1 fraction
Q8VDN2ATPase, Na+/K+ transporting, alpha 1 polypeptideAtp1a1MS11317.04Total brain
Q6PIE5ATPase, Na+/K+ transporting, alpha 2 polypeptideAtp1a2MS11217.05Total brain
P06687ATPase, Na+/K+ transporting, alpha 3 polypeptideAtp1a3MS11212.010LS1 fraction
Q9R0K7ATPase, Ca2+ transporting, plasma membrane 2Atp2b2MS1334.03Total brain
Q03265ATP synthase alpha subunit, isoform 1Atp5a1MS6021.012LS1 fraction
P56480 (P10719)ATP synthase beta subunitAtp5bMS5613.05LS1 fraction, total brain
P62815V-type proton ATPase subunit B, brain isoformAtp6v1b2MS5736.014LS1 fraction
Q6PCU2ATPase, H+ transporting, lysosomal V1 subunit E1Atp6v1e1MS2615.04LS1 fraction
P47728Calbindin 2Calb2MS3114.03LS1 fraction
P28480T-complex protein 1 subunit alphaTcp1MS6011.05LS1 fraction
Q5XIM9T-complex protein 1 subunit betaCct2MS5718.06LS1 fraction
Q68FD5Clathrin, heavy polypeptide (Hc)CltcMS19224.028Total brain
P63041Complexin 1Cplx1MS1517.02LS1 fraction
P39053Dynamin 1Dnm1MS, IP988.07Total brain
Q62952Dihydropyrimidinase-like 3Dpysl3MS6231.07LS1 fraction
P38650Dynein cytoplasmic 1 heavy chain 1Dync1h1MS53218.04LS1 fraction
P85845Fascin homologFscn1MS549.03LS1 fraction
P50398Rab GDP dissociation inhibitor alphaGdi1MS5149.016LS1 fraction
P50399Rab GDP dissociation inhibitor betaGdi2MS5125.05LS1 fraction
P59215 (P18872)Guanine nucleotide-binding protein G(o) subunit alphaGnao1MS4012.03LS1 fraction, total brain
P07901Heat shock protein 90, alphaHsp90aa1MS, WB855.02Total brain
P11499Heat shock protein 90, betaHsp90ab1MS, WB837.02Total brain
O35814Stress-induced phosphoprotein 1Stip1MS6310.04LS1 fraction
Q2PQA9Kinesin-1 heavy chainKif5bMS1104.03LS1 fraction
Q9WV63Kinesin-like protein KIF2AKif2aMS805.03LS1 fraction
P63086Mitogen-activated protein kinase 1Mapk1MS4120.06LS1 fraction
Q3B8Q0Microtubule-associated protein RP/EB family member 2Mapre2MS3711.02LS1 fraction
P19332Microtubule-associated protein tauMaptMS767.05LS1 fraction
P34926Microtubule-associated protein 1AMap1aMS3002.03LS1 fraction
P15205Microtubule-associated protein 1BMap1bMS2702.05LS1 fraction
P15146 (P20357)Microtubule-associated protein 2Map2MS1996.06LS1 fraction, total brain
Q9QYF3Myosin VAMyo5aMS2125.08LS1 fraction
Q63610Tropomyosin alpha-3Tpm3MS2919.04LS1 fraction
P85969Beta-soluble NSF attachment proteinNapbMS3411.03LS1 fraction
Q9Z0E0NeurochondrinNcdnMS794.02LS1 fraction
P46460N-Ethylmaleimide sensitive fusion proteinNsfMS, WB, IP8310.06Total brain
Q9QXU9ProSAASPcsk1nMS2718.05LS1 fraction
Q9EPC6Profilin 2Pfn2MS1518.03LS1 fraction
P60203Proteolipid protein (myelin) 1Plp1MS309.02LS1 fraction
P63329PP2B catalytic subunit alpha isoformPpp3caMS5928.011LS1 fraction
Q63716Peroxiredoxin 1Prdx1MS2220.03LS1 fraction
P35704Peroxiredoxin 2Prdx2MS2231.08LS1 fraction
O35244Similar to peroxiredoxin 6Prdx6MS2532.05LS1 fraction
P63011Ras-related protein Rab-3ARab3aWB2531.07Total brain
Q9WU34Neuron-specific septin-3Sept3MS4111.04LS1 fraction
Q9JJM9Septin 5Sept5MS4310.03LS1 fraction
Q9WVC0Septin 7Sept7MS5115.05LS1 fraction
O35179Endophilin-A1Sh3gl2MS4029.09LS1 fraction
Q5PPJ9Endophilin-B2Sh3glb2MS458.03LS1 fraction
P60881Synaptosome-associated protein 25Snap25MS, WB2314.02LS1 fraction
Q61548Synaptosome-associated protein 91Snap91MS924.03Total brain
P37377Synuclein, alphaSncaMS1439.03LS1 fraction
Q91ZZ3Synuclein, betaSncbMS1450.05LS1 fraction
P16546Spectrin alpha 2Spna2MS2854.09Total brain
Q62261Spectrin beta 2Spnb2MS2741.02Total brain
P6303960-kDa heat shock proteinHspd1MS6146.025LS1 fraction
O35814Stress-induced phosphoprotein 1Stip1MS6310.04LS1 fraction
P13668StathminStmn1MS1736.05LS1 fraction
P61264Syntaxin 1BStx1bMS3313.03Total brain
P61765 (O08599)Syntaxin-binding protein 1Stxbp1MS, IP6819.010LS1 fraction, total brain
Q9JIS5Synaptic vesicle glycoprotein 2ASv2aMS835.02Total brain
O88935Synapsin-1Syn1WB, IP7516.05Total brain
Q63537 (Q64332)Synapsin-2Syn2WB, IP6317.07LS1 fraction, total brain
Q62910Synaptojanin 1Synj1MS1734.05LS1 fraction
Q6P9V9 (Q99KA2)Tubulin alpha-1B chainTuba1bMS, WB, IP5045.021LS1 fraction, total brain
Q5XIF6Tubulin alpha-4A chainTuba4aMS5036.05LS1 fraction
P85108Tubulin beta-2A chainTubb2aMS5064.02LS1 fraction
Q3KRE8Tubulin beta-2B chainTubb2bMS5064.010LS1 fraction
Q4QRB4Tubulin beta-3 chainTubb3bMS5066.012LS1 fraction
Q6P9T8Tubulin beta-4B chainTubb4bMS5063.028LS1 fraction
P69897Tubulin beta-5 chainTubb5bMS5063.06LS1 fraction
Q00981Ubiquitin carboxyl-terminal hydrolase isozyme L1Uchl1MS2537.06LS1 fraction
P63045Vesicle-associated membrane protein 2Vamp2MS, WB, IP1346.06LS1 fraction
P3521314-3-3 protein beta polypeptideYwhabMS2830.02LS1 fraction
P6225914-3-3 protein epsilon polypeptideYwhaeMS2916.02Total brain
P6851114-3-3 protein eta polypeptideYwhahMS2852.07LS1 fraction
P6825514-3-3 protein thetaYwhaqMS2830.04LS1 fraction
  • a UniProtKB/Swiss-Prot accession numbers.

  • b Interactors were found by immunoprecipitation of endogenous LRRK2 (IP) or LRRK2 WD40 pulldown assays analyzed by Western blotting (WB) and mass spectrometry (MS).

  • c Sequence coverage for proteins identified by LC-MS/MS.

  • d Number of identified unique peptides.

  • e Lysate from adult mouse brain (total brain) or the LS1 fraction.