TABLE 2

Starvation-induced germ cell death in different genetic backgrounds

Experimental group and treatmentaAvg no. of corpses/gonad ± SEfPg
ControlStarvation
lin-35(n745) animalsb
    N2; EP(RNAi)*0.36 ± 0.05 (103)0.90 ± 0.11 (83)0.0002
    N2; ced-1(RNAi)7.07 ± 0.31 (109)15.83 ± 0.61 (108)<0.0001
    lin-35(n745); EP(RNAi)0.13 ± 0.06 (38)0.13 ± 0.05 (45)0.9876
    lin-35(n745); ced-1(RNAi)3.42 ± 0.34 (45)2.14 ± 0.26 (51)0.0057
    ced-1::GFP; EP(RNAi)*6.11 ± 0.21 (135)13.27 ± 0.36 (133)<0.0001
    ced-1::GFP; lin-35(RNAi)3.98 ± 0.20 (41)3.15 ± 0.17 (40)0.0002
ced-9(RNAi) animalsc
    N2; ced-9(RNAi)*3.1 ± 0.18 (21)3.9 ± 0.21 (21)0.2102
    N2; EP/ced-1(RNAi)5.65 ± 0.23 (40)12.66 ± 0.34 (34)<0.0001
    N2; ced-1/ced-9(RNAi)11.05 ± 0.35 (41)12.74 ± 0.32 (42)0.0002
    lin-35(n745); EP/ced-1(RNAi)3.15 ± 0.15 (40)2.19 ± 0.16 (31)0.0013
    lin-35(n745); ced-1/ced-9(RNAi)10.22 ± 0.43 (44)12 ± 0.48 (44)0.0048
dpl-1 animalsd
    dpl-1(n3643); EP(RNAi)0.35 ± 0.08 (37)0.22 ± 0.06 (37)0.1962
    dpl-1(n3643); ced-1(RNAi)3.69 ± 0.27 (42)3.39 ± 0.21 (49)0.4304
    ced-1::GFP; dpl-1(RNAi)*5.76 ± 0.25 (29)5.33 ± 0.26 (30)0.2116
efl-1(seI)V and efl-2(RNAi) animalse
    efl-1(seI)V; EP(RNAi)0.14 ± 0.06 (36)0.33 ± 0.11 (36)0.1962
    efl-1(seI)V; ced-1(RNAi)8.47 ± 0.33 (38)19.9 ± 0.36 (40)<0.0001
    ced-1::GFP; efl-2(RNAi)*4.98 ± 0.24 (63)11.44 ± 0.43 (63)<0.0001
  • a One-day-old adult animals with different genetic backgrounds were subjected to starvation and observed under Nomarski or fluorescence microscopy to detect corpses (see details in Materials and Methods). EP, empty plasmid. *, controls.

  • b lin-35(n745) animals did not respond to starvation-induced germ cell apoptosis.

  • c ced-9(RNAi) animals did not show evidence of starvation-induced germ cell apoptosis.

  • d DPL-1 is required to induce apoptosis under starvation.

  • e efl-1(seI)V and efl-2(RNAi) animals did respond to starvation-induced apoptosis.

  • f The number of observed gonads (one per animal) is shown in parentheses. These values represent at least two independent experiments.

  • g The P values were determined using an unpaired t test (Mann-Whitney); a P value of < 0.0001 was considered significant.