TABLE 1

Primers for site-directed mutagenesis

MutationDirectionaSequence (5′–3′)b
K14RFGGCCAGCGTTGTCTCCAGACTTTTTTTCAGCTGGACC
RGGTCCAGCTGAAAAAAAGTCTGGAGACAACGCTGGCC
K68RFGGCTTCAAAGAAAAATCCTAGACTCATTAATGCCCTTCGGCG
RCGCCGAAGGGCATTAATGAGTCTAGGATTTTTCTTTGAAGCC
K710RFCCAATCAACTCTATACGAAGATTTTCCATTGTGCAAAAG
RCTTTTGCACAATGGAAAATCTTCGTATAGAGTTGATTGG
K716RFGAAAATTTTCCATTGTGCAAAGGACTCCCTTACAAATGAATGG
RCCATTCATTTGTAAGGGAGTCCTTTGCACAATGGAAAATTTTC
K710/716RFCCAATCAACTCTATACGAAGATTTTCCATTGTGCAAAGGACTCCCTTACAAATGAATGG
RCCATTCATTTGTAAGGGAGTCCTTTGCACAATGGAAAATCTTCGTATAGAGTTGATTGG
K1041RFCCTCACAGCAATTCAGACAACTGGAATCTGAAG
RCTTCAGATTCCAGTTGTCTGAGTTGCTGTGAGG
K1080RFGAAACTCTGTTCCACAGAGCTCTGAATTTACATAC
RGTATGTAAATTCAGAGCTCTGTGGAACAGAGTTTC
K1218RFGATCTCACAGCAAGATACACAGAAGG
RCCTTCTGTGTATCTTGCTGTGAGATC
  • a F, forward; R, reverse.

  • b The codons at the sites for K-to-R mutations are underlined.