TABLE 2

Information for all spectra from three different trialsa

ResiduebPeptideMS methodUnmodified mass (Da)Observed m/zy and b ion coverage
K68*(Formyl)NPK(UGG)LINALRMS/MS1,179.69590.82+y1-y7, b2-b5, b7
K68(Formyl)NPK(UGG)LINALRMRM-MS1,179.69590.82+y1-y7, b2-b5
K710*K(UGG)FSIVQK(CAM)TPLQ(Dea)M(Oxi)NGIEEDSDEPLERMS/MS3,090.48773.64+y1-y10, b2-b3, b5-b11
K710(Formyl)K(UGG)FSIVQKMRM-MS990.56496.32+y1-y6, b2-b6
K710/K716*K(UGG)FSIVQK(UGG)TPLQM(Oxi)N(Dea)GIEEDSDEPLERMS/MS3,147.50787.94+y1, y3-y11, y13, b3-b8, b10
K716*(Formyl)FSIVQK(UGG)TPLQMNGIEEDSDEPLERMRM-MS2,916.40973.13+y1-y6, y8-y10, b2-b7, b9-b10
K716KFSIVQK(UGG)TPLQM(Oxi)N(Dea)GIEEDSDEPLERMS/MS3,033.46750.44+y1-y10, y12-y13, b2, b4-b8, b10-b13
K716(Formyl)FSIVQK(UGG)TPLQMNGIEEDSDEPLERMS/MS2,933.40978.83+y1-y2, y4-y5, y7-y12, b3-b7, b9
K716(Formyl)FSIVQK(UGG)MRM-MS862.47432.22+y1-y5, b2-b4
K1041*(Formyl)AYFLQTSQQLK(UGG)QLESEGRMRM-MS2,267.14756.73+y1-y9, b2-b4
K1041AYFLQTSQQLK(UGG)QLESEGRMS/MS2,239.12747.43+y1-y14, b2-b6, b8-b11
K1041AYFLQTSQQLK(UGG)QLESEGRMS/MS2,239.13747.43+y4-y13
K1080*(Gln→Pyro-Glu)QPYFETLFHK(UGG)MS/MS1,405.67703.82+y2-y8, b2-b6
  • a All spectra achieved a Protein Pilot confidence score of ≥98.

  • b Representative spectra are shown in Fig. 1 for proteins marked with an asterisk.