TABLE 1

Yeast strains used in this study

StrainGenotypeReference or source
BY4741MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0Yeast deletion collection (Open Biosystems, Inc.)
9arrΔ (EN60)ecm21Δ::KANMX csr2Δ::KANMX bsd2Δ rog3Δ::NATMX rod1Δ ygr068cΔ aly2Δ aly1Δ ldb19Δ ylr392cΔ::HIS3 his3 leu2Δ0 ura3Δ058
ldb19ΔMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 ldb19Δ::KANMX4Yeast deletion collection (Open Biosystems, Inc.)
rod1Δ rog3Δ (JT5858)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1Δ::KANMX4 rog3Δ::KANMX4This study
aly1Δ aly2Δ (D2-6A)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 aly1Δ::KANMX4 aly2Δ::KANMX486
ecm21Δ csr2Δ (JT6751)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 ecm21Δ::KANMX4 csr2Δ::KANMX4This study
art5Δ rim8Δ (JT5860)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 art5Δ::KANMX4 rim8Δ::KANMX4This study
art10ΔMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 art10Δ::KANMX4Yeast deletion collection (Open Biosystems, Inc.)
rod1Δ rog3Δ ldb19Δ (JT6675)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1Δ::KANMX4 rog3Δ::KANMX4 ldb19Δ::NATMXThis study
bar1Δ (JT5915)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 bar1Δ::CgLEU2This study
ldb19Δ bar1Δ (JT5916)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 ldb19Δ::KANMX4 bar1Δ::CgLEU2This study
rod1Δ rog3Δ bar1Δ (JT5917)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1Δ::KANMX4 rog3Δ::KANMX4 bar1Δ::CgLEU2This study
rod1Δ rog3Δ ldb19Δ bar1Δ (JT6674)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1Δ::KANMX4 rog3Δ::KANMX4 ldb19Δ::NATMX bar1Δ::CgLEU2This study
sst2Δ (JT6755)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 sst2Δ::SpHIS5This study
ldb19Δ sst2Δ (JT6660)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 ldb19Δ::KANMX4 sst2Δ::SpHIS5This study
rod1Δ rog3Δ sst2Δ (JT6702)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1Δ::KANMX4 rog3Δ::KANMX4 sst2Δ::SpHIS5This study
rod1Δ rog3Δ ldb19Δ sst2Δ (JT6662)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1Δ::KANMX4 rog3Δ::KANMX4 ldb19Δ::NATMX sst2Δ::SpHIS5This study
sst2Δ GEV (JT5919)bMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 sst2Δ::SpHIS5 leu2Δ0::GEV::NATMXThis study
rod1Δ rog3Δ ldb19Δ sst2Δ GEV (JT6716)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1Δ::KANMX4 rog3Δ::KANMX4 ldb19Δ::NATMX sst2Δ::SpHIS5 leu2Δ0::GEV::NATMXThis study
STE2-mCherry bar1Δ (JT6677)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 bar1Δ::CgLEU2This study
STE2-mCherry ldb19Δ bar1Δ (JT6678)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 ldb19Δ::KANMX4 bar1Δ::CgLEU2This study
STE2-mCherry rod1Δ rog3Δ bar1Δ (JT6679)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 rod1Δ::KANMX4 rog3Δ::KANMX4 bar1Δ::CgLEU2This study
STE2-mCherry rod1Δ rog3Δ ldb19Δ bar1Δ (JT6680)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-mCherry::URA3 rod1Δ::KANMX4 rog3Δ::KANMX4 ldb19Δ::NATMX bar1Δ::CgLEU2This study
STE2-GFP (JT6757)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-GFP::HPHThis study
9arrΔ STE2-GFP (JT6758)ecm21::G418 csr2::G418 bsd2 rog3::NATMX rod1 ygr068c aly2 aly1 ldb19 ylr392c::HIS his3 leu2Δ0 ura3Δ0 STE2-GFP::HPHThis study
ldb19Δ STE2-GFP (JT6759)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 ldb19Δ::KANMX4 STE2-GFP::HPHThis study
rod1Δ rog3Δ STE2-GFP (JT6760)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-GFP::HPH rod1Δ::KANMX4 rog3Δ::KANMX4This study
rod1Δ rog3Δ ldb19Δ STE2-GFP (JT6761)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-GFP::HPH rod1Δ::KANMX4 rog3Δ::KANMX4 ldb19Δ::NATMXThis study
aly1Δ aly2Δ STE2-GFP (JT6762)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 STE2-GFP::HPH aly1Δ::KANMX4 aly2Δ::KANMX4This study
bar1Δ FUS1prom-eGFP (JT6686)aMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 bar1Δ::URA3 FUS1prom-eGFP::LEU2This study
rod1Δ rog3Δ ldb19Δ bar1Δ FUS1prom-eGFP (JT6668)aMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 rod1Δ::KANMX4 rog3Δ::KANMX4 ldb19Δ::NATMX bar1Δ::URA3 FUS1prom-eGFP::LEU2This study
BJ5459MATa ura3-52 trp1 lys2-801 leu2Δ1 his3Δ200 pep4Δ::HIS3 prb1Δ1.6R can1 GAL cir+160
BJ5459 GEV (JT6743)bMATa ura3-52 trp1 lys2-801 leu2Δ1 his3Δ200 pep4Δ::HIS3 prb1Δ1.6R can1 GAL leu2Δ1::GEV::NATMXThis study
cnb1ΔMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 cnb1Δ::KANMX4Yeast deletion collection (Open Biosystems, Inc.)
cna1Δ cna2Δ (JT5574)MATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 cna1Δ::KANMX4 cna1Δ::KANMX4116
JRY11MATa ura3-52 trp1 lys2-801 leu21 his3200 pep4::HIS3 prb11.6R can1 GAL CNA2-S-TEV-ZZ-KANrMX6 CNA1-GFP-LEU2116
cnb1Δ GEV (JT6694)bMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NATMX cnb1Δ::KANMX4This study
cna1Δ cna2Δ GEV (JT6695)bMATa leu2Δ0 ura3Δ0 his3Δ1 met15Δ0 leu2Δ0::GEV::NATMX cna1Δ::KANMX4 cna1Δ::KANMX4This study
  • a To generate strains with the integrated FUS1prom-eGFP reporter, the cassette (73) was amplified by PCR and introduced by DNA-mediated transformation into a bar1Δ derivative of BY4741. The resulting strain (JT6686) was mated to a MATα lbd19Δ rod1Δ rog3Δ bar1Δ mutant. The resulting diploids were sporulated, and MATa ldb19Δ rod1Δ rog3Δ ldb19Δ bar1Δ FUS1prom-eGFP spores were identified after tetrad dissection.

  • b To generate a GEV-expressing version of the indicated yeast strain, pACT1-GEV (69, 161) was digested with EcoRV and introduced into the cells of interest by DNA-mediated transformation (65), and nourseothricin (NAT)-resistant colonies were selected in which GEV (expressed under the control of an ACT1 promoter) is integrated at the leu2Δ0 locus.