TABLE 1

Plasmids and yeast strains used in this study

Plasmid or yeast strainGenotypeaReference or source
Plasmids
    pC1818sc LEU2 FL-FUN1228–396)6
    pC1818-5Asc LEU2 FL-FUN12-5A28–396)This study
    YEpW-FUN12hc TRP1 FUN12This study
    YEpW-FUN12-5Ahc TRP1 FUN12-5AThis study
    YCpU-RPP1Asc URA3 RPP1AThis study
    YCpW-RPP2Bsc TRP1 RPP2BThis study
Yeast strains
    W303aMATa leu2-3,112 trp1-1 his3-11,15 ura3-1 ade2-1 can1-10013
    D56MATa rpl1BΔ::TRP1 rpp2BΔ::HIS3 ura3-1 leu2-3,112 can1-10013
    D57MATa rpp1AΔ::LEU2 rpp2BΔ::HIS3 ura3-1 trp1-1 can1-10013
    J111MATa ura3-52 leu2-3 leu2-112 fun12::hisG48
    KAY761MATa ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1 can1-100 rdnΔΔ::HIS3 pNOY373 [2μ RDN LEU2]52
    KAY968MATa ade2-1 his3-11 leu2-3,112 ura3-1 trp1-1 can1-100 rdnΔΔ::HIS3 pNOY373-40 [2μ rdn-40 LEU2]35
  • a sc, single copy; hc, high copy number.