TABLE 2.

Stimulation of Rev1 activity by the Polη peptide encompassing residues 510 to 632 (Rad30-3)

Template nucleotidedNTP addedEnzymekcat (min−1)Km (μM−1)kca t /Km (min−1/μM−1)Relative efficiency
GdCTPRev11.26 ± 0.20.048 ± 0.0126.31
Rev1 + Rad30-33.94 ± 0.30.039 ± 0.0061013.84
ApadCTPRev10.98 ± 0.051.15 ± 0.20.851
Rev1 + Rad30-33.44 ± 0.30.87 ± 0.083.854.65
  • a AP, abasic site.