TABLE 1.

Steady-state kinetic analyses of nucleotide incorporation opposite undamaged templates A and G and opposite an abasic site by the Rev1 and Rev1-Polη* complexes

Template nucleotidedNTP addedEnzymebkcat (min1)Km (μM)kcat/Km (min−1 μM−1)Relative efficiency
AdCTPRev11.02 ± 0.086.08 ± 1.40.171
Rev1-Polη*4.1 ± 0.345.83 ± 1.30.74.1
APadCTPRev11.87 ± 0.090.81 ± 0.22.31
Rev1-Polη*8.24 ± 0.541.06 ± 0.37.773.4
GdGTPRev14.31 ± 0.412 ± 2.40.361
Rev1-Polη*15.26 ± 0.87.6 ± 125.6
GdATPRev11.88 ± 0.3192.6 ± 310.011
Rev1-Polη*7.98 ± 0.9186 ± 17.20.0434.3
GdTTPRev12.45 ± 0.294.5 ± 240.0261
Rev1-Polη*8.65 ± 1.145.4 ± 110.197.3
GdCTPRev13.16 ± 0.240.045 ± 0.00270.21
Rev1-Polη*13.71 ± 0.40.032 ± 0.0054286.1
  • a AP, abasic site.

  • b *, catalytically inactive Polη in the Rev1-Polη* complex.