Table 1.

Yeast strains used in this study

StrainGenotypeExtrachromosomal plasmid(s)
TK161-R2V (CAPΔN)a MAT a his3 leu2 trp1 ura3 ade8 ras2::LEU2 RAS2Val-19 cap::pCAPΔN-1
TK36-1a MATα his3 leu2 trp1 ura3 ade8 cyr1-2 ras2::LEU2 YEP24-ADC1-CYR1
TS5 MATα his3 leu2 trp1 ura3 ade8 cyr1-2 ras2::LEU2 YEP24-ADC1-CYR1(606–1764)
FS1a MAT a his3 leu2 trp1 ura3 ade8 can1 cap::pCAPΔN-1
FS20 MAT a his3 leu2 trp1 ura3 ade8 can1 pAD4-FLAG-CAP
FS56 MAT a his3 leu2 trp1 ura3 ade8 can1 cap::pCAPΔN-1 pAD4-FLAG-CAP(L13P/E28V)
FS57 MAT a his3 leu2 trp1 ura3 ade8 can1 cap::pCAPΔN-1 pAD4-FLAG-CAP(N12S/E28G)
FS55 MAT a his3 leu2 trp1 ura3 ade8 can1 cap::pCAPΔN-1 pAD4-FLAG-CAP(R26G)
FS66 MAT a his3 leu2 trp1 ura3 ade8 can1 cap::pCAPΔN-1 pAD4-FLAG-CAP, pAS2-1-
GST-CYR1(1764–2026)
FS68 MAT a his3 leu2 trp1 ura3 ade8 can1 cap::pCAPΔN-1 pAD4-FLAG-CAP(L13P/E28V), pAS2-1-GST-CYR1(1764–2026)
FS69 MAT a his3 leu2 trp1 ura3 ade8 can1 cap::pCAPΔN-1 pAD4-FLAG-CAP(N12S/E28G), pAS2-1-GST-CYR1(1764–2026)
FS70 MAT a his3 leu2 trp1 ura3 ade8 can1 cap::pCAPΔN-1 pAD4-FLAG-CAP(R26G), pAS2-1-GST-CYR1(1764–2026)
Y187–207 MATα his3-200 leu2-3,112 trp1-901 ura3-52 ade2-101 canR gal4Δ metgal80Δ ura3::GALUAS-GAL1tata-lacZ cap::pCAPΔN-2 pGAD-CYR1(1898–2026)
  • a These strains were described previously (25, 37, 43).