Table 3.

Two-Hybrid interactions between Pan1p, End3p, and Sla1pa

Plasmidβ-Galactosidase activity (U)
pGAD424pSLA1ΔSRpSLA1-SRpSLA1-SRΔNPFpPAN1-LR2
pGBT9<1<1<1<1<1
pPAN1-LR1<1<117 ± 814 ± 6ND
pPAN1-CT<1<1<1NDND
pEND3<1<158 ± 1242 ± 9216 ± 23
pEND3-EH<1<193 ± 1579 ± 19<1
pEND3-ER<1<1<1ND136 ± 18
  • a The indicated pairs of plasmids were introduced into SFY526 cells, and β-galactosidase activity (average ± standard deviations for at least three transformants) was determined as described in Materials and Methods. ND, not determined.