Table 2.

FACS analysis for detection of DNA degradation

Time (h)% Sub-G1 DNA contenta
Wild typeVector143a175h245c248w273h305m
243.67 ± 1.21.98 ± 1.02.01 ± 0.812.05 ± 0.912.40 ± 0.731.89 ± 1.02.14 ± 0.403.68 ± 2.40
4812.53 ± 1.304.69 ± 1.603.58 ± 0.935.04 ± 1.203.79 ± 1.904.07 ± 1.404.02 ± 1.605.65 ± 1.50
7220.44 ± 2.605.24 ± 1.905.78 ± 0.834.85 ± 1.305.60 ± 1.705.78 ± 1.304.98 ± 1.806.18 ± 1.80
  • a Expression vectors bearing the indicated cDNAs were transiently transfected into Saos-2 cells. Values are the average percent sub-G1 DNA content were recorded at each time point posttransfection. Values shown are the averages of three separate experiments.