Table 1.

Quantitation of apoptosis by TUNEL staininga

Constructp53-induced apoptosisCI
Wild type34.100.28–0.34
143a13.080.03–0.07
175h13.230.04–0.07
245c13.390.04–0.07
248w12.530.03–0.06
273h13.360.04–0.07
305m18.220.07–0.12
325v18.280.07–0.12
  • a Expression vectors bearing the indicated cDNAs were transiently transfected into Saos-2 cells, and the cells were stained for p53 expression and TUNEL. p53-induced apoptosis was calculated as the percentage of TUNEL-positive cells of all cells expressing the indicated p53 cDNA. The results of three separate experiments were subjected to a two-variance statistical analysis compared to wild-type p53 to arrive at a confidence interval (CI) andP value. The P value for each sample was <0.0005 and thus considered statistically significantly different from the value for wild-type p53.